Can the SCD test and terminal uridine nick-end labeling by flow cytometry technique (TUNEL/FCM) be used interchangeably to measure sperm DNA damage in routine laboratory practice?
نویسندگان
چکیده
منابع مشابه
Longitudinal study of sperm DNA fragmentation as measured by terminal uridine nick end-labelling assay.
BACKGROUND One major limitation in the use of sperm DNA fragmentation as measured by the TdT (terminal deoxynucleotidyl transferase)-mediated dUTP nick-end labelling (TUNEL) assay is the paucity of solid data on the stability of this parameter. METHODS The objective of our study was to evaluate variations in the degree of sperm DNA fragmentation, as measured by the TUNEL assay, over a 6 month...
متن کاملDetection of DNA damage in prokaryotes by terminal deoxyribonucleotide transferase-mediated dUTP nick end labeling.
Numerous agents can damage the DNA of prokaryotes in the environment (e.g., reactive oxygen species, irradiation, and secondary metabolites such as antibiotics, enzymes, starvation, etc.). The large number of potential DNA-damaging agents, as well as their diverse modes of action, precludes a simple test of DNA damage based on detection of nucleic acid breakdown products. In this study, free 3'...
متن کاملDiagnostic accuracy of sperm chromatin dispersion test to evaluate sperm deoxyribonucleic acid damage in men with unexplained infertility.
OBJECTIVE To compare the sperm chromatin dispersion (SCD) test and the terminal uridine nick-end labeling (TUNEL) assay for assessment of sperm DNA damage. DESIGN Prospective comparative experimental study. SETTING Andrology laboratory. PATIENT(S) Twenty subfertile men with unexplained infertility. INTERVENTION(S) Sperm DNA damage was determined in the same semen samples using the TUNEL...
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متن کاملEffects of Sperm DNA Fragmentation on Semen Parameters and ICSI Outcome Determined by an Improved SCD Test, Halosperm
Background Sperm DNA fragmentation is known as an important cause of male infertility. The influence of sperm DNA damage on reproductive potential has been subject of many studies indicating various results and remaining the subject controversial. In this study, we investigated differences of the semen parameters and intracytoplasmic sperm injection (ICSI) outcome according to sperm DNA fragmen...
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ژورنال
عنوان ژورنال: Basic and Clinical Andrology
سال: 2019
ISSN: 2051-4190
DOI: 10.1186/s12610-019-0098-2